Dataset Information

Cloning and Expression of a New Trichinella spiralis Serine Protease and Its Role in Invading Host Intestinal Epithelium.

ABSTRACT:

Background

In previous studies, a new Trichinella spiralis serine protease 1.1 (TsSP1.1) was identified in surface proteins of T. spiralis muscle larvae (ML) by proteomics analysis, but its functions in T. spiralis infection are unknown. The aim of this study was to investigate the roles of TsSP1.1 during larval intrusion of gut epithelium.

Methods

From January 2019 to March 2021, complete TsSP1.1 cDNA sequence was cloned and expressed in Escherichia coli BL21 at the Department of Parasitology, Medical College of Zhengzhou University, Zhengzhou, China. Expression and location of TsSP1.1 in the parasite were investigated using indirect immunofluorescence assay (IIFA) and Western blotting. The in vitro intestinal epithelium cells (IECs) intrusion assay was used to ascertain the roles of TsSP1.1 during larval intrusion of IECs and gut epithelium.

Results

TsSP1.1 was a surface and secretory protein, which was expressed at various T. spiralis stages, and principally localized at cuticle, stichosome and embryos of the nematode. rTsSP1.1 accelerated larval intrusion of IECs, whereas anti-rTsSP1.1 antibodies impeded larval intrusion. The acceleration and inhibtion was dose-dependently related to rTsSP1.1 and anti-TsSP1.1 antibodies. Block of the IIL with anti-rTsSP1.1 serum also impeded larval intrusion of gut mucosa.

Conclusion

TsSP1.1 participates in T. spiralis intrusion of gut epithelium.

SUBMITTER: Liu F

PROVIDER: S-EPMC9682372 | biostudies-literature | 2022 Jul-Sep

REPOSITORIES: biostudies-literature

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Publications

Cloning and Expression of a New <i>Trichinella spiralis</i> Serine Protease and Its Role in Invading Host Intestinal Epithelium.

Liu Fang F Song Yan Yan YY Zhang Ru R Liu Ruo Dan RD Jiang Peng P Cui Jing J Wang Zhong Quan ZQ

Iranian journal of parasitology 20220701 3

<h4>Background</h4>In previous studies, a new <i>Trichinella spiralis</i> serine protease 1.1 (TsSP1.1) was identified in surface proteins of <i>T. spiralis</i> muscle larvae (ML) by proteomics analysis, but its functions in <i>T. spiralis</i> infection are unknown. The aim of this study was to investigate the roles of TsSP1.1 during larval intrusion of gut epithelium.<h4>Methods</h4>From January 2019 to March 2021, complete TsSP1.1 cDNA sequence was cloned and expressed in <i>Escherichia coli</ ...[more]

PMID: 36466014

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