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Defining the S-Glutathionylation Proteome by Biochemical and Mass Spectrometric Approaches.

ABSTRACT: Protein S-glutathionylation (SSG) is a reversible post-translational modification (PTM) featuring the conjugation of glutathione to a protein cysteine thiol. SSG can alter protein structure, activity, subcellular localization, and interaction with small molecules and other proteins. Thus, it plays a critical role in redox signaling and regulation in various physiological activities and pathological events. In this review, we summarize current biochemical and analytical approaches for characterizing SSG at both the proteome level and at individual protein levels. To illustrate the mechanism underlying SSG-mediated redox regulation, we highlight recent examples of functional and structural consequences of SSG modifications. Finally, we discuss the analytical challenges in characterizing SSG and the thiol PTM landscape, future directions for understanding of the role of SSG in redox signaling and regulation and its interplay with other PTMs, and the potential role of computational approaches to accelerate functional discovery.

SUBMITTER: Li X

PROVIDER: S-EPMC9687251 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Defining the S-Glutathionylation Proteome by Biochemical and Mass Spectrometric Approaches.

Li Xiaolu X Zhang Tong T Day Nicholas J NJ Feng Song S Gaffrey Matthew J MJ Qian Wei-Jun WJ

Antioxidants (Basel, Switzerland) 20221117 11

Protein S-glutathionylation (SSG) is a reversible post-translational modification (PTM) featuring the conjugation of glutathione to a protein cysteine thiol. SSG can alter protein structure, activity, subcellular localization, and interaction with small molecules and other proteins. Thus, it plays a critical role in redox signaling and regulation in various physiological activities and pathological events. In this review, we summarize current biochemical and analytical approaches for characteriz ...[more]

PMID: 36421458

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Dataset Information

Defining the S-Glutathionylation Proteome by Biochemical and Mass Spectrometric Approaches.

Publications

Defining the S-Glutathionylation Proteome by Biochemical and Mass Spectrometric Approaches.

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