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Eukaryotic CRFK Cells Motion Characterized with Atomic Force Microscopy


ABSTRACT: We performed a time-lapse imaging with atomic force microscopy (AFM) of the motion of eukaryotic CRFK (Crandell-Rees Feline Kidney) cells adhered onto a glass surface and anchored to other cells in culture medium at 37 °C. The main finding is a gradient in the spring constant of the actomyosin cortex along the cells axis. The rigidity increases at the rear of the cells during motion. This observation as well as a dramatic decrease of the volume suggests that cells may organize a dissymmetry in the skeleton network to expulse water and drive actively the rear edge.

SUBMITTER: Zamora-Ceballos M 

PROVIDER: S-EPMC9692694 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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