Ontology highlight
ABSTRACT: Aims
Our objective is to identify first-trimester plasmatic miRNAs associated with and predictive of GDM.Methods
We quantified miRNA using next-generation sequencing in discovery (Gen3G: n = 443/GDM = 56) and replication (3D: n = 139/GDM = 76) cohorts. We have diagnosed GDM using a 75-g oral glucose tolerance test and the IADPSG criteria. We applied stepwise logistic regression analysis among replicated miRNAs to build prediction models.Results
We identified 17 miRNAs associated with GDM development in both cohorts. The prediction performance of hsa-miR-517a-3p|hsa-miR-517b-3p, hsa-miR-218-5p, and hsa-let7a-3p was slightly better than GDM classic risk factors (age, BMI, familial history of type 2 diabetes, history of GDM or macrosomia, and HbA1c) (AUC 0.78 vs. 0.75). MiRNAs and GDM classic risk factors together further improved the prediction values [AUC 0.84 (95% CI 0.73-0.94)]. These results were replicated in 3D, although weaker predictive values were obtained. We suggest very low and higher risk GDM thresholds, which could be used to identify women who could do without a diagnostic test for GDM and women most likely to benefit from an early GDM prevention program.Conclusions
In summary, three miRNAs combined with classic GDM risk factors provide excellent prediction values, potentially strong enough to improve early detection and prevention of GDM.
SUBMITTER: Legare C
PROVIDER: S-EPMC9693764 | biostudies-literature | 2022
REPOSITORIES: biostudies-literature
Légaré Cécilia C Desgagné Véronique V Thibeault Kathrine K White Frédérique F Clément Andrée-Anne AA Poirier Cédrik C Luo Zhong Cheng ZC Scott Michelle S MS Jacques Pierre-Étienne PÉ Perron Patrice P Guérin Renée R Hivert Marie-France MF Bouchard Luigi L
Frontiers in endocrinology 20221111
<h4>Aims</h4>Our objective is to identify first-trimester plasmatic miRNAs associated with and predictive of GDM.<h4>Methods</h4>We quantified miRNA using next-generation sequencing in discovery (Gen3G: n = 443/GDM = 56) and replication (3D: n = 139/GDM = 76) cohorts. We have diagnosed GDM using a 75-g oral glucose tolerance test and the IADPSG criteria. We applied stepwise logistic regression analysis among replicated miRNAs to build prediction models.<h4>Results</h4>We identified 17 miRNAs ass ...[more]