Dataset Information

A Capsid Structure of Ralstonia solanacearum podoviridae GP4 with a Triangulation Number T = 9.

ABSTRACT: GP4, a new Ralstonia solanacearum phage, is a short-tailed phage. Few structures of Ralstonia solanacearum phages have been resolved to near-atomic resolution until now. Here, we present a 3.7 Å resolution structure of the GP4 head by cryo-electron microscopy (cryo-EM). The GP4 head contains 540 copies of major capsid protein (MCP) gp2 and 540 copies of cement protein (CP) gp1 arranged in an icosahedral shell with a triangulation number T = 9. The structures of gp2 and gp1 show a canonical HK97-like fold and an Ig-like fold, respectively. The trimeric CPs stick on the surface of the head along the quasi-threefold axis of the icosahedron generating a sandwiched three-layer electrostatic complementary potential, thereby enhancing the head stability. The assembly pattern of the GP4 head provides a platform for the further exploration of the interaction between Ralstonia solanacearum and corresponding phages.

SUBMITTER: Zheng J

PROVIDER: S-EPMC9698820 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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A Capsid Structure of Ralstonia solanacearum podoviridae GP4 with a Triangulation Number T = 9.

Zheng Jing J Chen Wenyuan W Xiao Hao H Yang Fan F Li Xiaowu X Song Jingdong J Cheng Lingpeng L Liu Hongrong H

Viruses 20221101 11

GP4, a new Ralstonia solanacearum phage, is a short-tailed phage. Few structures of Ralstonia solanacearum phages have been resolved to near-atomic resolution until now. Here, we present a 3.7 Å resolution structure of the GP4 head by cryo-electron microscopy (cryo-EM). The GP4 head contains 540 copies of major capsid protein (MCP) gp2 and 540 copies of cement protein (CP) gp1 arranged in an icosahedral shell with a triangulation number T = 9. The structures of gp2 and gp1 show a c ...[more]

PMID: 36366529

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Project description:Following a request from the European Commission, the EFSA Panel on Plant Health performed a pest categorisation of the Ralstonia solanacearum species complex (RSSC), a distinguishable cosmopolitan group of bacterial plant pathogens (including R. solanacearum, Ralstonia pseudosolanacearum and two subspecies of Ralstonia syzygii) of the family Burkholderiaceae. The RSSC causes bacterial wilt in solanaceous crops, such as potato, tomato and pepper, but can also cause wilts in other important food crops such as fruit banana, plantain banana and cassava. The pest survives in the soil, and a number of weed species can also be infected by the pest, often asymptomatically. The RSSC is regulated in Council Directive 2000/29/EC (Annex IAII) (indicated by its former name R. solanacearum, as delimited by Yabuuchi et al.) as a harmful organism whose introduction into the EU is banned. In addition, Council Directive 1998/57/EC (amended by Commission Directive 2006/63/CE) concerns the measures to be taken within EU Member States (MS) against the RSSC to (a) detect it and determine its distribution, (b) prevent its occurrence and spread, and (c) control it with the aim of eradication. The pest is present in several EU MS, but in all cases with a restricted distribution and under official control. New phylotypes of the RSSC could enter the EU primarily via host plants for planting (including seed tubers). The pest could establish in the EU, as climatic conditions are favourable, hosts are common and the pathogen has high adaptability. Spread is mainly via plants for planting. Substantial crop losses in the EU would occur in the presence of RSSC epidemics. The RSSC is regarded as one of the world's most important phytopathogenic bacteria due to its broad geographical distribution, large host range, aggressiveness, genetic diversity and long persistence in soil and water. The list of hosts and commodities for which the pest is regulated is incomplete due to the high diversity of hosts and the lack of knowledge of the complete host range. Moreover, the comparative epidemiology of the different pathogen species has not yet been studied. The criteria assessed by the Panel for consideration of the RSSC as potential quarantine pest are met, while, for regulated non-quarantine pests, the criterion on the widespread presence in the EU is not met.

Dataset Information

A Capsid Structure of Ralstonia solanacearum podoviridae GP4 with a Triangulation Number T = 9.

Publications

A Capsid Structure of <i>Ralstonia solanacearum</i> <i>podoviridae</i> GP4 with a Triangulation Number T = 9.

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Dataset Information

A Capsid Structure of Ralstonia solanacearum podoviridae GP4 with a Triangulation Number T = 9.

Publications

A Capsid Structure of <i>Ralstonia solanacearum</i>&nbsp;<i>podoviridae</i> GP4 with a Triangulation Number T = 9.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

A Capsid Structure of <i>Ralstonia solanacearum</i> <i>podoviridae</i> GP4 with a Triangulation Number T = 9.