Normalization of NPM1 mutant transcript to the wild-type transcript.
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ABSTRACT:
SUBMITTER: Jennings LJ
PROVIDER: S-EPMC9713034 | biostudies-literature | 2022 Nov
REPOSITORIES: biostudies-literature
Publications
Normalization of <i>NPM1</i> mutant transcript to the wild-type transcript.
EJHaem 20220927 4
Current guidelines recommend that Acute Myeloid Leukemia (AML) patients with <i>NPM1</i> mutations should be monitored for measurable residual disease by quantifying the transcripts and normalizing them to <i>ABL1</i> transcripts. In this short report, a simple and highly accurate method to quantify the <i>NPM1</i> mutant transcript normalized to the wild-type <i>NPM1</i> transcript is presented. The percent mutant transcript correlates very well to the corresponding mutant allele frequency as d ...[more]
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