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Genome sequence of pineapple secovirus B, a second sadwavirus reported infecting Ananas comosus.

ABSTRACT: The complete genome sequence of pineapple secovirus B (PSV-B), a new virus infecting pineapple (Ananas comosus) on the island of Oahu, Hawaii, was determined by high-throughput sequencing (HTS). The genome comprises two RNAs that are 5,956 and 3,808 nt long, excluding the 3'-end poly-A tails, both coding for a single large polyprotein. The RNA1 polyprotein contains five conserved domains associated with replication, while the RNA2 polyprotein is cleaved into the movement protein and coat protein. PSV-B is representative of a new species in the subgenus Cholivirus (genus Sadwavirus; family Secoviridae), as the level of amino acid sequence identity to recognized members of this subgenus in the Pro-Pol and coat protein regions is below currently valid species demarcation thresholds.

SUBMITTER: Larrea-Sarmiento A

PROVIDER: S-EPMC9741570 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Genome sequence of pineapple secovirus B, a second sadwavirus reported infecting Ananas comosus.

Larrea-Sarmiento Adriana A Geering Andrew D W ADW Olmedo-Velarde Alejandro A Wang Xupeng X Borth Wayne W Matsumoto Tracie K TK Suzuki Jon Y JY Wall Marisa M MM Melzer Michael M Moyle Richard R Sharman Murray M Hu John J Thomas John E JE

Archives of virology 20221021 12

The complete genome sequence of pineapple secovirus B (PSV-B), a new virus infecting pineapple (Ananas comosus) on the island of Oahu, Hawaii, was determined by high-throughput sequencing (HTS). The genome comprises two RNAs that are 5,956 and 3,808 nt long, excluding the 3'-end poly-A tails, both coding for a single large polyprotein. The RNA1 polyprotein contains five conserved domains associated with replication, while the RNA2 polyprotein is cleaved into the movement protein and coat protein ...[more]

PMID: 36269415

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Cloning and expression analysis of a PISTILLATA homologous gene from pineapple (Ananas comosus L. Merr).

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