Dataset Information

Efficient purification of flavonoids from bamboo shoot residues of Phyllostachys edulis by macroporous resin and their hypoglycemic activity.

ABSTRACT: The adsorption/desorption process of flavonoids obtained from bamboo shoots residues of Phyllostachys edulis (RPEFs) was established by resin screening, adsorption isotherms, adsorption kinetics and dynamic tests. Results indicated the Langmuir equation fairly fitted the isotherms data and the pseudo-second-order model accurately described its kinetic adsorption on HPD-500 resin. The optimal process was as follows: 2 mg/mL of solution, 2.8 BV feed volume under 2 BV/h flow rate for adsorption; 5 BV volume of 70 % ethanol with a flow rate of 2 BV/h for desorption. After enrichment, the flavonoids in RPEFs increased from 1.39 % to 37.34 %. The analysis of UPLC-TOF-MS/MS uncovered that the purified RPEFs were composed of four major flavonoids. It had potent hypoglycemic activity via PI3K/AKT pathway by upregulating the protein expression of PI3K, AKT, IRS-1, and GLUT4 in a dose-dependent manner based on HepG2-IR cells. Those results provide possible utilization of Phyllostachys edulis by-products in food additives.

SUBMITTER: Wang Y

PROVIDER: S-EPMC9743288 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Efficient purification of flavonoids from bamboo shoot residues of <i>Phyllostachys edulis</i> by macroporous resin and their hypoglycemic activity.

Wang Yanbin Y Zhang Yalan Y Cheng Junwen J Zhao Jiancheng J Shi Rui R He Liang L Li Qin Q Chen Yongjian Y

Food chemistry: X 20221112

The adsorption/desorption process of flavonoids obtained from bamboo shoots residues of <i>Phyllostachys edulis</i> (RPEFs) was established by resin screening, adsorption isotherms, adsorption kinetics and dynamic tests. Results indicated the Langmuir equation fairly fitted the isotherms data and the pseudo-second-order model accurately described its kinetic adsorption on HPD-500 resin. The optimal process was as follows: 2 mg/mL of solution, 2.8 BV feed volume under 2 BV/h flow rate for adsorpt ...[more]

PMID: 36519103

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Project description:BackgroundAccording to the growth pattern of bamboo, sympodial bamboo and monopodial bamboo are considered as two mainly kinds of bamboo. They have different phenotypes and different characteristics in developmental stage. Much attention had been paid on the study of bamboo cultivation, processing, physiology, biochemistry and molecular biology, which had made great progresses in the last decade, especially for the highlighted achievement of the bamboo genomics. However, there is no information available on concerning comparative profiling of miRNAs between sympodial bamboo and monopodial bamboo, which might play important roles in the regulation of bamboo development.Methodology/principal findingsWe identified the profiles of small RNAs using leaf tissues from one sympodial bamboo i.e. moso bamboo (Phyllostachys edulis) and another monopodial bamboo i.e. ma bamboo (Dendrocalamus latiflorus). The result showed that there were 19,295,759 and 11,513,888 raw sequence reads, in which 92 and 69 conserved miRNAs, as well as 95 and 62 novel miRNAs were identified in moso bamboo and ma bamboo, respectively. The ratio of high conserved miRNA families in ma bamboo is more than that in moso bamboo. In addition, a total of 49 and 106 potential targets were predicted in moso bamboo and ma bamboo, respectively, in which several targets for novel miRNAs are transcription factors that play important roles in plant development. More importantly, annotation of differentially expressed target genes was performed based on the analysis of pathway and gene ontology terms enrichment.Conclusions/significanceThis study provides the first large-scale sight of discovery and comparative characterization of miRNAomes between two representative bamboos belonged to sympodial bamboo and monopodial bamboo, respectively. Although it will be necessary to validate the function of miRNAs through more experimental research in further, these results lay a foundation for unraveling the miRNA-mediated molecular processes in different kinds of bamboo.

Dataset Information

Efficient purification of flavonoids from bamboo shoot residues of Phyllostachys edulis by macroporous resin and their hypoglycemic activity.

Publications

Efficient purification of flavonoids from bamboo shoot residues of <i>Phyllostachys edulis</i> by macroporous resin and their hypoglycemic activity.

Similar Datasets

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