Ontology highlight
ABSTRACT:
SUBMITTER: Sclimenti CR
PROVIDER: S-EPMC97615 | biostudies-literature | 2001 Dec
REPOSITORIES: biostudies-literature
Sclimenti C R CR Thyagarajan B B Calos M P MP
Nucleic acids research 20011201 24
We previously established that a unidirectional site-specific recombinase, the phage phiC31 integrase, can mediate integration into mammalian chromosomes. The enzyme directs integration of plasmids bearing the phage attB recognition site into pseudo attP sites, a set of native sequences related to the phage attP recognition site. Here we use two cycles of DNA shuffling and screening in Escherichia coli to obtain evolved integrases that possess significant improvements in integration frequency an ...[more]