Ontology highlight
ABSTRACT:
SUBMITTER: Hong X
PROVIDER: S-EPMC9784400 | biostudies-literature | 2022 Oct
REPOSITORIES: biostudies-literature
Hong Xiao X Cholko Timothy T Chang Chia-En A CA Wheeldon Ian I
Chem catalysis 20221007 10
Biopolymer-scaffold modification is widely used to enhance enzyme catalysis. A central challenge is predicting the effects of scaffold position on enzyme properties. Here, we use a computational-experimental approach to develop a model for the effects of DNA scaffold position on enzyme kinetics. Using phosphotriesterase modified with a 20bp dsDNA, we demonstrate that conjugation position is as important as the scaffold's chemistry and structure. Multiscale simulations predict the effective subst ...[more]