Unknown

Dataset Information

0

Cross-Feeding and Enzymatic Catabolism for Mannan-Oligosaccharide Utilization by the Butyrate-Producing Gut Bacterium Roseburia hominis A2-183.


ABSTRACT: β-Mannan is abundant in the human diet and in hemicellulose derived from softwood. Linear or galactose-substituted β-mannan-oligosaccharides (MOS/GMOSs) derived from β-mannan are considered emerging prebiotics that could stimulate health-associated gut microbiota. However, the underlying mechanisms are not yet resolved. Therefore, this study investigated the cross-feeding and metabolic interactions between Bifidobacterium adolescentis ATCC 15703, an acetate producer, and Roseburia hominis A2-183 DSMZ 16839, a butyrate producer, during utilization of MOS/GMOSs. Cocultivation studies suggest that both strains coexist due to differential MOS/GMOS utilization, along with the cross-feeding of acetate from B. adolescentis E194a to R. hominis A2-183. The data suggest that R. hominis A2-183 efficiently utilizes MOS/GMOS in mono- and cocultivation. Notably, we observed the transcriptional upregulation of certain genes within a dedicated MOS/GMOS utilization locus (RhMosUL), and an exo-oligomannosidase (RhMan113A) gene located distally in the R. hominis A2-183 genome. Significantly, biochemical analysis of β-1,4 mannan-oligosaccharide phosphorylase (RhMOP130A), α-galactosidase (RhGal36A), and exo-oligomannosidase (RhMan113A) suggested their potential synergistic role in the initial utilization of MOS/GMOSs. Thus, our results enhance the understanding of MOS/GMOS utilization by potential health-promoting human gut microbiota and highlight the role of cross-feeding and metabolic interactions between two secondary mannan degraders inhabiting the same ecological niche in the gut.

SUBMITTER: Bhattacharya A 

PROVIDER: S-EPMC9784577 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Cross-Feeding and Enzymatic Catabolism for Mannan-Oligosaccharide Utilization by the Butyrate-Producing Gut Bacterium <i>Roseburia hominis</i> A2-183.

Bhattacharya Abhishek A   Majtorp Lovisa L   Birgersson Simon S   Wiemann Mathias M   Sreenivas Krishnan K   Verbrugghe Phebe P   Van Aken Olivier O   Van Niel Ed W J EWJ   Stålbrand Henrik H  

Microorganisms 20221216 12


β-Mannan is abundant in the human diet and in hemicellulose derived from softwood. Linear or galactose-substituted β-mannan-oligosaccharides (MOS/GMOSs) derived from β-mannan are considered emerging prebiotics that could stimulate health-associated gut microbiota. However, the underlying mechanisms are not yet resolved. Therefore, this study investigated the cross-feeding and metabolic interactions between <i>Bifidobacterium adolescentis</i> ATCC 15703, an acetate producer, and <i>Roseburia homi  ...[more]

Similar Datasets

| PRJEA68655 | ENA
| PRJNA33399 | ENA
| PRJNA68431 | ENA
| PRJNA46157 | ENA
| S-EPMC4645204 | biostudies-literature
| S-EPMC5622956 | biostudies-literature
| S-EPMC8746519 | biostudies-literature
| S-EPMC9787297 | biostudies-literature
| S-EPMC7600330 | biostudies-literature
| PRJNA656617 | ENA