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Identification of Genomic Variants of SARS-CoV-2 Using Nanopore Sequencing.


ABSTRACT: Background and Objectives: SARS-CoV-2 is the first global threat and life-changing event of the twenty-first century. Although efficient treatments and vaccines have been developed, due to the virus's ability to mutate in key regions of the genome, whole viral genome sequencing is needed for efficient monitoring, evaluation of the spread, and even the adjustment of the molecular diagnostic assays. Materials and Methods: In this study, Nanopore and Ion Torrent sequencing technologies were used to detect the main SARS-CoV-2 circulating strains in Timis County, Romania, between February 2021 and May 2022. Results: We identified 22 virus lineages belonging to seven clades: 20A, 20I (Alpha, V1), 21B (Kappa), 21I (Delta), 21J (Delta), 21K (Omicron), and 21L (Omicron). Conclusions: Results obtained with both methods are comparable, and we confirm the utility of Nanopore sequencing in large-scale epidemiological surveillance due to the lower cost and reduced time for library preparation.

SUBMITTER: Capraru ID 

PROVIDER: S-EPMC9788413 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Identification of Genomic Variants of SARS-CoV-2 Using Nanopore Sequencing.

Capraru Ionut Dragos ID   Romanescu Mirabela M   Anghel Flavia Medana FM   Oancea Cristian C   Marian Catalin C   Sirbu Ioan Ovidiu IO   Chis Aimee Rodica AR   Ciordas Paula Diana PD  

Medicina (Kaunas, Lithuania) 20221215 12


<i>Background and Objectives</i>: SARS-CoV-2 is the first global threat and life-changing event of the twenty-first century. Although efficient treatments and vaccines have been developed, due to the virus's ability to mutate in key regions of the genome, whole viral genome sequencing is needed for efficient monitoring, evaluation of the spread, and even the adjustment of the molecular diagnostic assays. <i>Materials and Methods</i>: In this study, Nanopore and Ion Torrent sequencing technologie  ...[more]

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