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SCON-a Short Conditional intrON for conditional knockout with one-step zygote injection.

ABSTRACT: The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.

SUBMITTER: Wu SS

PROVIDER: S-EPMC9794761 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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SCON-a Short Conditional intrON for conditional knockout with one-step zygote injection.

Wu Szu-Hsien Sam SS Lee Heetak H Szép-Bakonyi Réka R Colozza Gabriele G Boese Ayse A Gert Krista R KR Hallay Natalia N Lee Ji-Hyun JH Kim Jihoon J Zhu Yi Y Linssen Margot M MM Pilat-Carotta Sandra S Hohenstein Peter P Theussl Hans-Christian HC Pauli Andrea A Koo Bon-Kyoung BK

Experimental & molecular medicine 20221209 12

The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging. ...[more]

PMID: 36494589

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