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Dataset from de novo transcriptome assembly of Myristica fatua leaves using MinION nanopore sequencer.

ABSTRACT: Myristica fatua is a tropical fruit tree species originating from Indonesia. Very few genomic resources are available for the species. We developed a full-length transcriptome assembly using long-read sequencing (MinION Nanopore technology) and produced 4.3 million reads (3.5 G of bases). The assembled full-length transcript was constructed using the RATTLE program and assembled 21,098 transcripts. The transcript ranged from 201 - 14,174 bp, and N50 was 2,017 bp. The transcripts were annotated with the UNIPROT database using BlastX. The functional annotation was performed using Blast2go software. The 8,445 microsatellite motif-containing contigs were identified. The raw reads are deposited in the ENA (European Nucleotide Archive) with ENA experiment accession number ERX6798613.

SUBMITTER: Matra DD

PROVIDER: S-EPMC9803912 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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Dataset from de novo transcriptome assembly of Myristica fatua leaves using MinION nanopore sequencer.

Matra Deden Derajat DD Adrian M M Karmanah Kusuma Jakty J Duminil Jérôme J Sobir Poerwanto Roedhy R

Data in brief 20221217

Myristica fatua is a tropical fruit tree species originating from Indonesia. Very few genomic resources are available for the species. We developed a full-length transcriptome assembly using long-read sequencing (MinION Nanopore technology) and produced 4.3 million reads (3.5 G of bases). The assembled full-length transcript was constructed using the RATTLE program and assembled 21,098 transcripts. The transcript ranged from 201 - 14,174 bp, and N50 was 2,017 bp. The transcripts were anno ...[more]

PMID: 36593766

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