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Pharokka: a fast scalable bacteriophage annotation tool.


ABSTRACT:

Summary

In recent years, there has been an increasing interest in bacteriophages, which has led to growing numbers of bacteriophage genomic sequences becoming available. Consequently, there is a need for a rapid and consistent genomic annotation tool dedicated for bacteriophages. Existing tools either are not designed specifically for bacteriophages or are web- and email-based and require significant manual curation, which makes their integration into bioinformatic pipelines challenging. Pharokka was created to provide a tool that annotates bacteriophage genomes easily, rapidly and consistently with standards compliant outputs. Moreover, Pharokka requires only two lines of code to install and use and takes under 5 min to run for an average 50-kb bacteriophage genome.

Availability and implementation

Pharokka is implemented in Python and is available as a bioconda package using 'conda install -c bioconda pharokka'. The source code is available on GitHub (https://github.com/gbouras13/pharokka). Pharokka has been tested on Linux-64 and MacOSX machines and on Windows using a Linux Virtual Machine.

SUBMITTER: Bouras G 

PROVIDER: S-EPMC9805569 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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Publications

Pharokka: a fast scalable bacteriophage annotation tool.

Bouras George G   Nepal Roshan R   Houtak Ghais G   Psaltis Alkis James AJ   Wormald Peter-John PJ   Vreugde Sarah S  

Bioinformatics (Oxford, England) 20230101 1


<h4>Summary</h4>In recent years, there has been an increasing interest in bacteriophages, which has led to growing numbers of bacteriophage genomic sequences becoming available. Consequently, there is a need for a rapid and consistent genomic annotation tool dedicated for bacteriophages. Existing tools either are not designed specifically for bacteriophages or are web- and email-based and require significant manual curation, which makes their integration into bioinformatic pipelines challenging.  ...[more]

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