Dataset Information

Evaluation of CD4⁺ cells infiltration as a prognostic factor in cervical intraepithelial neoplasia 2.

ABSTRACT:

Objective

To identify candidate predictors for the prognosis of cervical intraepithelial neoplasia 2 (CIN2) lesions and evaluate the prognostic value of the local immune response.

Methods

One hundred fifteen CIN2 patients were enrolled. The percentage of p16-, minichromosome maintenance complex component 2- or apolipoprotein B mRNA editing enzyme catalytic subunit 3G (APOBEC3G)-positive cells was determined immunohistochemically. Tumor-infiltrating lymphocytes (TILs) in intertumoral lesions were scored using an automated system. CIN3 disease progression and regression rates were estimated by the Kaplan-Meier method. A case-control study was conducted to screen CIN2 prognostic factors in 10 regression and 10 progression patients. Selected factors were examined in a cohort study to determine their prognostic value for CIN2.

Results

Among all participants, the cumulative progression and regression rates at 60 months were 0.477 and 0.510, respectively. In the case-control study, p16- and APOBEC3G-positive cells were higher in the progression group (p=0.043, p=0.023). Additionally, CD4⁺ cell infiltration was enhanced in the regression group (p=0.023). The cohort study revealed a significantly increased progression rate in patients with elevated p16-positive cells (p<0.001), and increased CD4⁺ TIL infiltration was associated with better regression (p=0.011). Kaplan-Meier analysis according to human papillomavirus (HPV) positivity revealed a greater CIN3 development risk in HPV16-positive patients than in HPV16-negative cases. Finally, multivariate analysis identified HPV16 infection and CD4⁺ TIL infiltration as independent prognostic factors in CIN2 regression.

Conclusion

CD4⁺ TIL infiltration in intertumoral lesions was related with CIN2 regression. Our findings suggest CD4⁺ TIL infiltration may be useful for the triage of CIN2 patients.

SUBMITTER: Chen G

PROVIDER: S-EPMC9807366 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Evaluation of CD4<sup>+</sup> cells infiltration as a prognostic factor in cervical intraepithelial neoplasia 2.

Chen Guanliang G Iwata Takashi T Sugawara Masaki M Nishio Hiroshi H Katoh Yuki Y Kukimoto Iwao I Aoki Daisuke D

Journal of gynecologic oncology 20221004 1

<h4>Objective</h4>To identify candidate predictors for the prognosis of cervical intraepithelial neoplasia 2 (CIN2) lesions and evaluate the prognostic value of the local immune response.<h4>Methods</h4>One hundred fifteen CIN2 patients were enrolled. The percentage of p16-, minichromosome maintenance complex component 2- or apolipoprotein B mRNA editing enzyme catalytic subunit 3G (APOBEC3G)-positive cells was determined immunohistochemically. Tumor-infiltrating lymphocytes (TILs) in intertumor ...[more]

PMID: 36245223

Similar Datasets

Project description:Cervical cancer is caused by human papillomavirus (HPV). The disease develops over many years through a series of precancerous lesions. Cervical cancer can be prevented by HPV-vaccination, screening and treatment of precancer before development of cervical cancer. The treatment of high-grade cervical dysplasia (CIN 2+) has traditionally been by cervical conization. Surgical procedures are associated with increased risk of undesirable side effects including bleeding, infection, scarring (stenosis), infertility and complications in later pregnancies. An inexpensive, non-invasive method of delivering therapeutics locally will be favorable to treat precancerous cervical lesions without damaging healthy tissue. The feasibility and safety of a sustained, continuous drug-releasing cervical polymeric implant for use in clinical trials was studied using a large animal model. The goat (Capra hircus), non-pregnant adult female Boer goats, was chosen due to similarities in cervical dimensions to the human. Estrus was induced with progesterone CIDR® vaginal implants for 14days followed by the administration of chorionic gonadotropins 48h prior to removal of the progesterone implants to relax the cervix to allow for the placement of the cervical implant. Cervical implants, containing 2% and 4% withaferin A (WFA), with 8 coats of blank polymer, provided sustained release for a long duration and were used for the animal study. The 'mushroom'-shaped cervical polymeric implant, originally designed for women required redesigning to be accommodated within the goat cervix. The cervical implants were well tolerated by the animals with no obvious evidence of discomfort, systemic or local inflammation or toxicity. In addition, we developed a new method to analyze tissue WFA levels by solvent extractions and LS/MS-MS. WFA was found to be localized to the target and adjacent tissues with 12-16ng WFA/g tissue, with essentially no detectable WFA in distant tissues. This study suggests that the goat is a good large animal model for the future development and evaluation of therapeutic efficacy of continuous local drug delivery by cervical polymeric implants to treat precancerous cervical lesions.

Dataset Information

Evaluation of CD4⁺ cells infiltration as a prognostic factor in cervical intraepithelial neoplasia 2.

Objective

Methods

Results

Conclusion

Publications

Evaluation of CD4<sup>+</sup> cells infiltration as a prognostic factor in cervical intraepithelial neoplasia 2.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Evaluation of CD4+ cells infiltration as a prognostic factor in cervical intraepithelial neoplasia 2.

Objective

Methods

Results

Conclusion

Publications

Evaluation of CD4<sup>+</sup> cells infiltration as a prognostic factor in cervical intraepithelial neoplasia 2.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Evaluation of CD4⁺ cells infiltration as a prognostic factor in cervical intraepithelial neoplasia 2.