Ontology highlight
ABSTRACT:
SUBMITTER: Collon K
PROVIDER: S-EPMC9808795 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
Collon Kevin K Gallo Matthew C MC Bell Jennifer A JA Chang Stephanie W SW Rodman John Croom Sueiro JCS Sugiyama Osamu O Kohn Donald B DB Lieberman Jay R JR
Human gene therapy 20221201 23-24
Lentiviral transduction of human mesenchymal stem cells (MSCs) induces long-term transgene expression and holds great promise for multiple gene therapy applications. Polybrene is the most commonly used reagent to improve viral gene transfer efficiency in laboratory research; however, it is not approved for human use and has also been shown to impair MSC proliferation and differentiation. Therefore, there is a need for optimized transduction protocols that can also be adapted to clinical settings ...[more]