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Tunable 2D diffusion of DNA nanostructures on lipid membranes.

ABSTRACT: DNA nanotechnology facilitates the synthesis of biomimetic models for studying biological systems. This work uses lipid bilayers as platforms for two-dimensional single-particle tracking of the dynamics of DNA nanostructures. Three different DNA origami structures adhere to the membrane through hybridization with cholesterol-modified strands. Their two-dimensional diffusion coefficient is modulated by changing the concentration of monovalent and divalent salts and the number of anchors. In addition, the diffusion coefficient is tuned by targeting cholesterol-modified anchor strands with strand-displacement reactions. We demonstrate a responsive system with changing diffusivity by selectively displacing membrane-bound anchor strands. We also show the programmed release of origami structures from the lipid membranes.

SUBMITTER: Bogawat Y

PROVIDER: S-EPMC9811667 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Tunable 2D diffusion of DNA nanostructures on lipid membranes.

Bogawat Yash Y Krishnan Swati S Simmel Friedrich C FC Santiago Ibon I

Biophysical journal 20221014 24

DNA nanotechnology facilitates the synthesis of biomimetic models for studying biological systems. This work uses lipid bilayers as platforms for two-dimensional single-particle tracking of the dynamics of DNA nanostructures. Three different DNA origami structures adhere to the membrane through hybridization with cholesterol-modified strands. Their two-dimensional diffusion coefficient is modulated by changing the concentration of monovalent and divalent salts and the number of anchors. In addit ...[more]

PMID: 36243925

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