Ontology highlight
ABSTRACT:
SUBMITTER: Terradas G
PROVIDER: S-EPMC9837192 | biostudies-literature | 2023 Jan
REPOSITORIES: biostudies-literature
Terradas Gerard G Bennett Jared B JB Li Zhiqian Z Marshall John M JM Bier Ethan E
Nature communications 20230112 1
The core components of CRISPR-based gene drives, Cas9 and guide RNA (gRNA), either can be linked within a self-contained single cassette (full gene-drive, fGD) or be provided in two separate elements (split gene-drive, sGD), the latter offering greater control options. We previously engineered split systems that could be converted genetically into autonomous full drives. Here, we examine such dual systems inserted at the spo11 locus that are recoded to restore gene function and thus organismic f ...[more]