Ontology highlight
ABSTRACT:
SUBMITTER: Li C
PROVIDER: S-EPMC9841435 | biostudies-literature | 2023 Jan
REPOSITORIES: biostudies-literature
Li Chuxuan C Zhu Hanwen H Jin Shikai S Maksoud Leora M LM Jain Nikhil N Sun Ji J Gao Yang Y
Nucleic acids research 20230101 1
DNA polymerase θ (Pol θ) plays an essential role in the microhomology-mediated end joining (MMEJ) pathway for repairing DNA double-strand breaks. However, the mechanisms by which Pol θ recognizes microhomologous DNA ends and performs low-fidelity DNA synthesis remain unclear. Here, we present cryo-electron microscope structures of the polymerase domain of Lates calcarifer Pol θ with long and short duplex DNA at up to 2.4 Å resolution. Interestingly, Pol θ binds to long and short DNA substrates s ...[more]