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Data on 2D culture characterisation of potential markers in human HER2-positive breast cancer cell lines.

ABSTRACT: To obtain this dataset, two human HER2-positive breast cancer cell lines (SKBR3 and MDA-MB-453 cell lines) were cultured in basal growth media to 80% confluence. Cells were passaged and total RNA extracted, RNA converted to cDNA and diluted to a working concentration of 40 ng/µL. Gene expression panels of cancer markers including Fibroblast growth factors (FGF), FGF receptors (FGFRs), cyclin-dependent kinases, cytokeratins, and WNT pathway components were then examined using Q-PCR. Gene expression was normalised against the expression of the endogenous gene 18S. This article describes the data used in the research article "Syndecan-4 regulates the HER2-positive breast cancer cell proliferation cells via CK19/AKT signaling" [1]. The data presented demonstrates the range of gene expression profiles of these cells and aims to provide more detail of all gene expression changes observed in these cell lines.

SUBMITTER: Pham SH

PROVIDER: S-EPMC9852922 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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Data on 2D culture characterisation of potential markers in human HER2-positive breast cancer cell lines.

Pham Son H SH Griffiths Lyn R LR Okolicsanyi Rachel K RK Haupt Larisa M LM

Data in brief 20230107

To obtain this dataset, two human HER2-positive breast cancer cell lines (SKBR3 and MDA-MB-453 cell lines) were cultured in basal growth media to 80% confluence. Cells were passaged and total RNA extracted, RNA converted to cDNA and diluted to a working concentration of 40 ng/µL. Gene expression panels of cancer markers including Fibroblast growth factors (<i>FGF</i>), FGF receptors (<i>FGFRs</i>), cyclin-dependent kinases, cytokeratins, and WNT pathway components were then examined using Q-PCR. ...[more]

PMID: 36687151

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