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Clock genes and environmental cues coordinate Anopheles pheromone synthesis, swarming, and mating.

ABSTRACT: Anopheles mating is initiated by the swarming of males at dusk followed by females flying into the swarm. Here, we show that mosquito swarming and mating are coordinately guided by clock genes, light, and temperature. Transcriptome analysis shows up-regulation of the clock genes period (per) and timeless (tim) in the head of field-caught swarming Anopheles coluzzii males. Knockdown of per and tim expression affects Anopheles gambiae s.s. and Anopheles stephensi male mating in the laboratory, and it reduces male An. coluzzii swarming and mating under semifield conditions. Light and temperature affect mosquito mating, possibly by modulating per and/or tim expression. Moreover, the desaturase gene desat1 is up-regulated and rhythmically expressed in the heads of swarming males and regulates the production of cuticular hydrocarbons, including heptacosane, which stimulates mating activity.

SUBMITTER: Wang G

PROVIDER: S-EPMC9854397 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Clock genes and environmental cues coordinate Anopheles pheromone synthesis, swarming, and mating.

Wang Guandong G Vega-Rodríguez Joel J Diabate Abdoulaye A Liu Jingnan J Cui Chunlai C Nignan Charles C Dong Ling L Li Fang F Ouedrago Cheick Oumar CO Bandaogo Abdoul Malik AM Sawadogo Péguédwindé Simon PS Maiga Hamidou H Alves E Silva Thiago Luiz TL Pascini Tales Vicari TV Wang Sibao S Jacobs-Lorena Marcelo M

Science (New York, N.Y.) 20210101 6527

Anopheles mating is initiated by the swarming of males at dusk followed by females flying into the swarm. Here, we show that mosquito swarming and mating are coordinately guided by clock genes, light, and temperature. Transcriptome analysis shows up-regulation of the clock genes period (per) and timeless (tim) in the head of field-caught swarming Anopheles coluzzii males. Knockdown of per and tim expression affects Anopheles gambiae s.s. ...[more]

PMID: 33479155

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Project description:Swarming is a key part of the natural system of reproduction of anopheline mosquito populations, and a better understanding of swarming and mating systems in a targeted species in its natural habitat would contribute to better design control strategies with a greater chance of success. Our study investigated the monthly occurrence of swarming and the mating frequency (within swarms) of Anopheles arabiensis in Dioulassoba, Burkina Faso and their relationship with local environmental factors. Mosquitoes collected from swarms were described in terms of body size, recent sugar meal intake, and female repletion, insemination, and Plasmodium falciparum infection status. Swarms of An. arabiensis were found in each month of the year. Both start and end times of swarming varied significantly between months, correlating with the time of sunset. Swarming mostly started after or coincided with sunset from late July to early October but occurred before sunset from late October to early July. Swarming duration, the number of mosquitoes and mating pairs per swarm, and time to first mating were significantly different between months in an inverse relationship with the monthly rainfall. The number of mating pairs was strongly and positively correlated with swarm size. Almost all the females caught in copula were inseminated but a very few were blood fed; no P. falciparum infection was observed. Males caught in copula and in solo were similar in body size and in the proportion which had taken a recent sugar meal. Our investigations showed that An. arabiensis reproductive activities are most frequent during the dry season, suggesting either the species' preference for dry climatic conditions or a lack of available breeding sites during the rainy season due to the seasonal flooding in this area. Targeting interventions to kill mosquitoes in swarms or to achieve an over-flooding ratio of sterile males during the rainy season would increase their efficiency in reducing the population density of this vector.

Dataset Information

Clock genes and environmental cues coordinate Anopheles pheromone synthesis, swarming, and mating.

Publications

Clock genes and environmental cues coordinate <i>Anopheles</i> pheromone synthesis, swarming, and mating.

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