Unknown

Dataset Information

0

Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST - RE1-silencing transcription factor.

ABSTRACT: The RE1-Silencing Transcription factor (REST) is essential for neuronal differentiation. Here, we report the first 18.5-angstrom electron microscopy structure of human REST. The refined electron map suggests that REST forms a torus that can accommodate DNA double-helix in the central hole. Additionally, we quantitatively described REST binding to the canonical DNA sequence of the neuron-restrictive silencer element. We developed protocols for the expression and purification of full-length REST and the shortened variant REST-N62 produced by alternative splicing. We tested the mutual interaction of full-length REST and the splicing variant REST-N62. Revealed structure-function relationships of master neuronal repressor REST will allow finding new biological ways of prevention and treatment of neurodegenerative disorders and diseases.

SUBMITTER: Veverka P 

PROVIDER: S-EPMC9860152 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST - RE1-silencing transcription factor.

Veverka Pavel P   Brom Tomáš T   Janovič Tomáš T   Stojaspal Martin M   Pinkas Matyáš M   Nováček Jiří J   Hofr Ctirad C  

Computational and structural biotechnology journal 20221219

The RE1-Silencing Transcription factor (REST) is essential for neuronal differentiation. Here, we report the first 18.5-angstrom electron microscopy structure of human REST. The refined electron map suggests that REST forms a torus that can accommodate DNA double-helix in the central hole. Additionally, we quantitatively described REST binding to the canonical DNA sequence of the neuron-restrictive silencer element. We developed protocols for the expression and purification of full-length REST a  ...[more]

Similar Datasets

Unknown Regulation of RE1 protein silencing transcription factor (REST) expression by HIP1 protein interactor (HIPPI).
| S-EPMC3190832 | biostudies-literature
Unknown Phosphatase activity of small C-terminal domain phosphatase 1 (SCP1) controls the stability of the key neuronal regulator RE1-silencing transcription factor (REST).
| S-EPMC6204920 | biostudies-literature
Unknown RE1 silencing transcription factor (REST) negatively regulates ALL1-fused from chromosome 1q (AF1q) gene transcription.
| S-EPMC4560861 | biostudies-literature
Unknown REST selectively represses a subset of RE1-containing neuronal genes in mouse embryonic stem cells.
| S-EPMC2685939 | biostudies-literature
Unknown RE1-silencing Transcription Factor (REST) Is Required for Nuclear Reprogramming by Inhibiting Transforming Growth Factor β Signaling Pathway.
| S-EPMC5207159 | biostudies-literature
Unknown Prion protein is essential for the RE1 silencing transcription factor (REST)-dependent developmental switch in synaptic NMDA receptors.
| S-EPMC5945644 | biostudies-literature
Unknown The RE1 binding protein REST regulates oligodendrocyte differentiation.
| S-EPMC3565470 | biostudies-literature
Transcriptomics Spatial Transcriptomics correlated Electron Microscopy
2023-06-21 | GSE202638 | GEO
Unknown MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression.
| S-EPMC2631966 | biostudies-literature
Unknown EWS and RE1-Silencing Transcription Factor Inhibit Neuronal Phenotype Development and Oncogenic Transformation in Ewing Sarcoma.
| S-EPMC3782006 | biostudies-literature