Dataset Information

Efficient production of cembratriene-ol in Escherichia coli via systematic optimization.

ABSTRACT:

Background

The tobacco leaf-derived cembratriene-ol exhibits anti-insect effects, but its content in plants is scarce. Cembratriene-ol is difficult and inefficiently chemically synthesised due to its complex structure. Moreover, the titer of reported recombinant hosts producing cembratriene-ol was low and cannot be applied to industrial production.

Results

In this study, Pantoea ananatis geranylgeranyl diphosphate synthase (CrtE) and Nicotiana tabacum cembratriene-ol synthase (CBTS) were heterologously expressed to synthsize the cembratriene-ol in Escherichia coli. Overexpression of cbts*, the 1-deoxy-D-xylulose 5-phosphate synthase gene dxs, and isopentenyl diphosphate isomerase gene idi promoted the production of cembratriene-ol. The cembratriene-ol titer was 1.53-folds higher than that of E. coli Z17 due to the systematic regulation of ggpps, cbts*, dxs, and idi expression. The production of cembratriene-ol was boosted via the overexpression of genes ispA, ispD, and ispF. The production level of cembratriene-ol in the optimal medium at 72 h was 8.55-folds higher than that before fermentation optimisation. The cembratriene-ol titer in the 15-L fermenter reached 371.2 mg L^- 1, which was the highest titer reported.

Conclusion

In this study, the production of cembratriene-ol in E. coli was significantly enhanced via systematic optimization. It was suggested that the recombinant E. coli producing cembratriene-ol constructed in this study has potential for industrial production and applications.

SUBMITTER: Yang H

PROVIDER: S-EPMC9872381 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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Publications

Efficient production of cembratriene-ol in Escherichia coli via systematic optimization.

Yang Haiquan H Zhang Kunjie K Shen Wei W Chen Lei L Xia Yuanyuan Y Zou Wei W Cao Yu Y Chen Xianzhong X

Microbial cell factories 20230124 1

<h4>Background</h4>The tobacco leaf-derived cembratriene-ol exhibits anti-insect effects, but its content in plants is scarce. Cembratriene-ol is difficult and inefficiently chemically synthesised due to its complex structure. Moreover, the titer of reported recombinant hosts producing cembratriene-ol was low and cannot be applied to industrial production.<h4>Results</h4>In this study, Pantoea ananatis geranylgeranyl diphosphate synthase (CrtE) and Nicotiana tabacum cembratriene-ol synthase (CBT ...[more]

PMID: 36694175

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Dataset Information

Efficient production of cembratriene-ol in Escherichia coli via systematic optimization.

Background

Results

Conclusion

Publications

Efficient production of cembratriene-ol in Escherichia coli via systematic optimization.

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