Epitope mapping of neutralizing botulinum neurotoxin A antibodies by phage display.
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ABSTRACT:
SUBMITTER: Mullaney BP
PROVIDER: S-EPMC98787 | biostudies-literature | 2001 Oct
REPOSITORIES: biostudies-literature
Publications
Epitope mapping of neutralizing botulinum neurotoxin A antibodies by phage display.
Mullaney B P BP Pallavicini M G MG Marks J D JD
Infection and immunity 20011001 10
Single-chain antibodies neutralize activity and bind nonoverlapping epitopes of botulinum A neurotoxin. Two phage display epitope libraries were constructed from the 1.3 kb of binding domain cDNA. The minimal epitopes selected against the single-chain Fv-Fc antibodies correspond to conformational epitopes with amino acid residues 1115 to 1223 (S25), 1131 to 1264 (3D12), and 889 to 1294 (C25). ...[more]
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