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Genetic dissection of intercellular interactions in vivo by membrane-permeable protein.


ABSTRACT: Unraveling cell-cell interaction is fundamental to understanding many biological processes. To date, genetic tools for labeling neighboring cells in mammals are not available. Here, we developed a labeling strategy based on the Cre-induced intercellular labeling protein (CILP). Cre-expressing donor cells release a lipid-soluble and membrane-permeable fluorescent protein that is then taken up by recipient cells, enabling fluorescent labeling of neighboring cells. Using CILP, we specifically labeled endothelial cells surrounding a special population of hepatocytes in adult mice and revealed their distinct gene signatures. Our results highlight the potential of CILP as a platform to reveal cell-cell interactions and communications in vivo.

SUBMITTER: Zhang S 

PROVIDER: S-EPMC9910597 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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Genetic dissection of intercellular interactions in vivo by membrane-permeable protein.

Zhang Shaohua S   Zhang Qianyu Q   Liu Zixin Z   Liu Kuo K   He Lingjuan L   Lui Kathy O KO   Wang Lixin L   Zhou Bin B  

Proceedings of the National Academy of Sciences of the United States of America 20221227 1


Unraveling cell-cell interaction is fundamental to understanding many biological processes. To date, genetic tools for labeling neighboring cells in mammals are not available. Here, we developed a labeling strategy based on the Cre-induced intercellular labeling protein (CILP). Cre-expressing donor cells release a lipid-soluble and membrane-permeable fluorescent protein that is then taken up by recipient cells, enabling fluorescent labeling of neighboring cells. Using CILP, we specifically label  ...[more]

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