Emu: species-level microbial community profiling of full-length 16S rRNA Oxford Nanopore sequencing data.
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ABSTRACT:
SUBMITTER: Curry KD
PROVIDER: S-EPMC9939874 | biostudies-literature | 2022 Jul
REPOSITORIES: biostudies-literature
Publications
Emu: species-level microbial community profiling of full-length 16S rRNA Oxford Nanopore sequencing data.
Curry Kristen D KD Wang Qi Q Nute Michael G MG Tyshaieva Alona A Reeves Elizabeth E Soriano Sirena S Wu Qinglong Q Graeber Enid E Finzer Patrick P Mendling Werner W Savidge Tor T Villapol Sonia S Dilthey Alexander A Treangen Todd J TJ
Nature methods 20220630 7
16S ribosomal RNA-based analysis is the established standard for elucidating the composition of microbial communities. While short-read 16S rRNA analyses are largely confined to genus-level resolution at best, given that only a portion of the gene is sequenced, full-length 16S rRNA gene amplicon sequences have the potential to provide species-level accuracy. However, existing taxonomic identification algorithms are not optimized for the increased read length and error rate often observed in long ...[more]
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