Dataset Information

Genomic and phenotypic profiling of Staphylococcus aureus isolates from bovine mastitis for antibiotic resistance and intestinal infectivity.

ABSTRACT:

Background

Staphylococcus aureus is one of the prevalent etiological agents of contagious bovine mastitis, causing a significant economic burden on the global dairy industry. Given the emergence of antibiotic resistance (ABR) and possible zoonotic spillovers, S aureus from mastitic cattle pose threat to both veterinary and public health. Therefore, assessment of their ABR status and pathogenic translation in human infection models is crucial.

Results

In this study, 43 S. aureus isolates associated with bovine mastitis obtained from four different Canadian provinces (Alberta, Ontario, Quebec, and Atlantic provinces) were tested for ABR and virulence through phenotypic and genotypic profiling. All 43 isolates exhibited crucial virulence characteristics such as hemolysis, and biofilm formation, and six isolates from ST151, ST352, and ST8 categories showed ABR. Genes associated with ABR (tetK, tetM, aac6', norA, norB, lmrS, blaR, blaZ, etc.), toxin production (hla, hlab, lukD, etc.), adherence (fmbA, fnbB, clfA, clfB, icaABCD, etc.), and host immune invasion (spa, sbi, cap, adsA, etc.) were identified by analyzing whole-genome sequences. Although none of the isolates possessed human adaptation genes, both groups of ABR and antibiotic-susceptible isolates demonstrated intracellular invasion, colonization, infection, and death of human intestinal epithelial cells (Caco-2), and Caenorhabditis elegans. Notably, the susceptibilities of S. aureus towards antibiotics such as streptomycin, kanamycin, and ampicillin were altered when the bacteria were internalized in Caco-2 cells and C. elegans. Meanwhile, tetracycline, chloramphenicol, and ceftiofur were comparatively more effective with ≤ 2.5 log₁₀ reductions of intracellular S. aureus.

Conclusions

This study demonstrated the potential of S. aureus isolated from mastitis cows to possess virulence characteristics enabling invasion of intestinal cells thus calling for developing therapeutics capable of targeting drug-resistant intracellular pathogens for effective disease management.

SUBMITTER: Majumder S

PROVIDER: S-EPMC9940407 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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Publications

Genomic and phenotypic profiling of Staphylococcus aureus isolates from bovine mastitis for antibiotic resistance and intestinal infectivity.

Majumder Satwik S Sackey Trisha T Viau Charles C Park Soyoun S Xia Jianguo J Ronholm Jennifer J George Saji S

BMC microbiology 20230220 1

<h4>Background</h4>Staphylococcus aureus is one of the prevalent etiological agents of contagious bovine mastitis, causing a significant economic burden on the global dairy industry. Given the emergence of antibiotic resistance (ABR) and possible zoonotic spillovers, S aureus from mastitic cattle pose threat to both veterinary and public health. Therefore, assessment of their ABR status and pathogenic translation in human infection models is crucial.<h4>Results</h4>In this study, 43 S. aureus is ...[more]

PMID: 36803552

Similar Datasets

Project description:Staphylococcus (S.) aureus is a major cause of bovine mastitis and is notorious for its capacity to resist antibiotics, presenting substantial risks to both livestock and human health. The aim of this research was to assess the prevalence of S. aureus in bovine mastitis cases, as well as to examine their patterns of antimicrobial resistance and virulence genes contributing to mastitis in cattle. For this study, 120 milk samples were gathered from clinically mastitis affected cows across three districts in the northern part of Bangladesh. The detection and confirmation of S. aureus involved standard microbiological and biochemical techniques. The antibiotic sensitivity of the strains was evaluated using the disk diffusion method with a variety of antibiotics frequently used in veterinary settings. Furthermore, PCR was utilized to explore the presence of virulence genes linked to the pathogenicity of S. aureus. Findings revealed that out of the sampled cases, 56 strains of S. aureus were isolated, indicating a prevalence rate of 46.66 % in cases of clinical mastitis. The results revealed a diverse range of antibiotic resistance patterns among the isolates, with a notable prevalence of resistance to penicillin (100 %), ampicillin (85 %), amoxicillin (75 %), tetracycline (66 %), chlortetracycline (64 %), azithromycin (57 %), kanamycin (54 %), and gentamicin (50 %). 36 isolates out of 56 (64 %) were multidrug resistant in nature. Furthermore, virulence gene profiling identified the genes responsible for biofilm formation (bap), adhesion, inflammation and tissue damage (seb, pvl), and toxin production (hla and hlb), indicating the potential pathogenicity of the isolates. Notably, 12 isolates (21.42 %) harbored gene linked to methicillin resistance (mecA), raising concerns about the potential transmission of antimicrobial-resistant S. aureus strains from dairy cows to humans through the food chain. These findings underscore the critical importance of implementing stringent antimicrobial stewardship practices and surveillance measures in dairy farming to mitigate the dissemination of antibiotic resistance.

Dataset Information

Genomic and phenotypic profiling of Staphylococcus aureus isolates from bovine mastitis for antibiotic resistance and intestinal infectivity.

Background

Results

Conclusions

Publications

Genomic and phenotypic profiling of Staphylococcus aureus isolates from bovine mastitis for antibiotic resistance and intestinal infectivity.

Similar Datasets

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