Ontology highlight
ABSTRACT:
SUBMITTER: Mishra P
PROVIDER: S-EPMC99492 | biostudies-literature | 2001 May
REPOSITORIES: biostudies-literature
Mishra P P Park P K PK Drueckhammer D G DG
Journal of bacteriology 20010501 9
Dephosphocoenzyme A (dephospho-CoA) kinase catalyzes the final step in coenzyme A biosynthesis, the phosphorylation of the 3'-hydroxy group of the ribose sugar moiety. Wild-type dephospho-CoA kinase from Corynebacterium ammoniagenes was purified to homogeneity and subjected to N-terminal sequence analysis. A BLAST search identified a gene from Escherichia coli previously designated yacE encoding a highly homologous protein. Amplification of the gene and overexpression yielded recombinant dephosp ...[more]