Characterization of intragenic recombination in a hyperthermophilic archaeon via conjugational DNA exchange.
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ABSTRACT:
SUBMITTER: Reilly MS
PROVIDER: S-EPMC99513 | biostudies-literature | 2001 May
REPOSITORIES: biostudies-literature
Publications
Characterization of intragenic recombination in a hyperthermophilic archaeon via conjugational DNA exchange.
Journal of bacteriology 20010501 9
Sulfolobus acidocaldarius is so far the only hyperthermophilic archaeon in which genetic recombination can be assayed by conjugation and simple selections. Crosses among spontaneous pyr mutants were able to resolve closely spaced chromosomal mutations, identify deletions and rearrangements, and map mutations to a given deletion interval. Frameshift mutations in pyrE exerted polar effects that depressed orotidine-5'-monophosphate decarboxylase activity (encoded by pyrF), whereas base pair substit ...[more]
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