Dataset Information

High-Molecular-Weight Plasmids Carrying Carbapenemase Genes bla_NDM-1, bla_KPC-2, and bla_OXA-48 Coexisting in Clinical Klebsiella pneumoniae Strains of ST39.

ABSTRACT:

Background

Klebsiella pneumoniae, a member of the ESKAPE group of bacterial pathogens, has developed multi-antimicrobial resistance (AMR), including resistance to carbapenems, which has increased alarmingly due to the acquisition of carbapenemase genes located on specific plasmids.

Methods

Four clinical K. pneumoniae isolates were collected from four patients of a neuro-intensive care unit in Moscow, Russia, during the point prevalence survey. The AMR phenotype was estimated using the Vitec-2 instrument, and whole genome sequencing (WGS) was done using Illumina and Nanopore technologies.

Results

All strains were resistant to beta-lactams, nitrofurans, fluoroquinolones, sulfonamides, aminoglycosides, and tetracyclines. WGS analysis revealed that all strains were closely related to K. pneumoniae ST39, capsular type K-23, with 99.99% chromosome identity. The novelty of the study is the description of the strains carrying simultaneously three large plasmids of the IncHI1B, IncC, and IncFIB groups carrying the carbapenemase genes of three types, bla_OXA-48, bla_NDM-1, and bla_KPC-2, respectively. The first of them, highly identical in all strains, was a hybrid plasmid that combined two regions of the resistance genes (bla_OXA-48 and bla_TEM-1 + bla_CTX-M-15 + bla_OXA-1 + catB + qnrS1 + int1) and a region of the virulence genes (iucABCD, iutA, terC, and rmpA2::IS110).

Conclusion

The spread of K. pneumoniae strains carrying multiple plasmids conferring resistance even to last-resort antibiotics is of great clinical concern.

SUBMITTER: Kuzina ES

PROVIDER: S-EPMC9961262 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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Publications

High-Molecular-Weight Plasmids Carrying Carbapenemase Genes <i>bla</i><sub>NDM-1</sub>, <i>bla</i><sub>KPC-2</sub>, and <i>bla</i><sub>OXA-48</sub> Coexisting in Clinical <i>Klebsiella pneumoniae</i> Strains of ST39.

Kuzina Ekaterina S ES Kislichkina Angelina A AA Sizova Angelika A AA Skryabin Yury P YP Novikova Tatiana S TS Ershova Olga N ON Savin Ivan A IA Khokhlova Olga E OE Bogun Alexander G AG Fursova Nadezhda K NK

Microorganisms 20230211 2

<h4>Background</h4><i>Klebsiella pneumoniae,</i> a member of the ESKAPE group of bacterial pathogens, has developed multi-antimicrobial resistance (AMR), including resistance to carbapenems, which has increased alarmingly due to the acquisition of carbapenemase genes located on specific plasmids.<h4>Methods</h4>Four clinical <i>K. pneumoniae</i> isolates were collected from four patients of a neuro-intensive care unit in Moscow, Russia, during the point prevalence survey. The AMR phenotype was e ...[more]

PMID: 36838424

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Project description:Aim of this study was to genetically characterize two carbapenemase-producing Escherichia coli strains obtained from a pediatric patient affected by diarrhea, expressing OXA-181 and/or NDM-5 type enzymes. The above microorganisms were collected in the same Desenzano hospital (Northern Italy) where the bla NDM- 5 gene was detected for the first time in Italy 3 years ago. One strain (5P), belonged to sequence type ST405/ST477 (according to Pasture/Oxford schemes) and serotype O102:H6. It was characterized by a 130562 bp multi-replicon plasmid IncFII/IncFIA/IncFIB (pVSI_NDM-5) enclosing two main antibiotic resistance islands: (i) ARI-I, 10030 bp in size, carried genes coding for β-lactam- (bla OXA- 1, bla CTX-M- 15), fluoroquinolone/aminoglycoside- (aac(6')-lb-cr) and phenicol- resistance (catB3), (ii) ARI-II, 15326 bp in size, carried genes coding for sulfonamide- (sul1), β-lactam- (bla NDM- 5, bla TEM- 1 B), phenicol- (catB3), trimethoprim- (dfrA17), antiseptic- (qacEΔ1), and aminoglycoside- (aadA5, rmtB) resistance. The other isolate (5M), belonged to sequence type ST2659/ST759 and serotype O50/02:H18, and carried four plasmids: a 153866 bp multi-replicon IncFII/IncFIA/IncFIB (pISV_IncFII_NDM-5), an 89866 bp IncI1 plasmid, a 51480 bp IncX3 plasmid (pISV_IncX3_OXA181), and a 41143 bp IncI plasmid (pISV_IncI_CMY-42). pISV_IncFII_NDM-5 carried two main antibiotic resistance islands: (i) ARI-III, 12220 bp in size, carried genes coding for β-lactam- (bla OXA- 1), fluoroquinolone/aminoglycoside- (aac(6')-lb-cr), tetracycline- (tet(B)) and phenicol- resistance (catB3, catA1), and ii) ARI-IV, 26527 bp in size, carried determinants coding for macrolide- (erm(B), mph(A)), sulfonamide- (sul1), beta-lactam- (bla NDM- 5, bla TEM- 1 B), trimethoprim- (dfrA14, dfrA12), antiseptic- (qacEΔ1), and aminoglycoside- resistance (aadA5). pISV_IncI_CMY-42 harbored the bla CMY- 42 gene coding for beta-lactam resistance, pISV_IncX3_OXA181 harbored genes encoding fluoroquinolone- (qnrS1) and beta-lactams- resistance (bla OXA- 181). In conclusion, the detection of two different NDM-5 E. coli strains from a pediatric patient with a history of travel to the Far East countries strongly highlight an increasing trend and risk of importation from such areas.

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Dataset Information

High-Molecular-Weight Plasmids Carrying Carbapenemase Genes bla_NDM-1, bla_KPC-2, and bla_OXA-48 Coexisting in Clinical Klebsiella pneumoniae Strains of ST39.

Background

Methods

Results

Conclusion

Publications

High-Molecular-Weight Plasmids Carrying Carbapenemase Genes <i>bla</i><sub>NDM-1</sub>, <i>bla</i><sub>KPC-2</sub>, and <i>bla</i><sub>OXA-48</sub> Coexisting in Clinical <i>Klebsiella pneumoniae</i> Strains of ST39.

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OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

High-Molecular-Weight Plasmids Carrying Carbapenemase Genes blaNDM-1, blaKPC-2, and blaOXA-48 Coexisting in Clinical Klebsiella pneumoniae Strains of ST39.

Background

Methods

Results

Conclusion

Publications

High-Molecular-Weight Plasmids Carrying Carbapenemase Genes <i>bla</i><sub>NDM-1</sub>, <i>bla</i><sub>KPC-2</sub>, and <i>bla</i><sub>OXA-48</sub> Coexisting in Clinical <i>Klebsiella pneumoniae</i> Strains of ST39.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

High-Molecular-Weight Plasmids Carrying Carbapenemase Genes bla_NDM-1, bla_KPC-2, and bla_OXA-48 Coexisting in Clinical Klebsiella pneumoniae Strains of ST39.