Ratiometric Near-Infrared Fluorescence Liposome Nanoprobe for H₂S Detection In Vivo.

ABSTRACT: Accurate detection of H₂S is crucial to understanding the occurrence and development of H₂S-related diseases. However, the accurate and sensitive detection of H₂S in vivo still faces great challenges due to the characteristics of H₂S diffusion and short half-life. Herein, we report a H₂S-activatable ratiometric near-infrared (NIR) fluorescence liposome nanoprobe HS-CG by the thin-film hydration method. HS-CG shows "always on" fluorescence signal at 816 nm and low fluorescence signal at 728 nm; the NIR fluorescence ratio between 728 and 816 nm (F₇₂₈/F₈₁₆) is low. Upon reaction with H₂S, the fluorescence at 728 nm could be more rapidly turned on due to strong electrostatic interaction between enriched HS^- and positively charged 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine (DPPC) doped in the liposome nanoprobe HS-CG, resulting in a large enhancement of F₇₂₈/F₈₁₆, which allows for sensitive visualization of the tumor H₂S levels in vivo. This study demonstrates that this strategy of electrostatic adsorption between HS^- and positively charged molecules provides a new way to enhance the reaction rate of the probe and H₂S, thus serving as an effective platform for improving the sensitivity of imaging.

SUBMITTER: Wu L 

PROVIDER: S-EPMC9961796 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature