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Generalized transduction of small Yersinia enterocolitica plasmids.

ABSTRACT: To study phage-mediated gene transfer in Yersinia, the ability of Yersinia phages to transduce naturally occurring plasmids was investigated. The transduction experiments were performed with a temperate phage isolated from a pathogenic Yersinia enterocolitica strain and phage mixtures isolated from sewage. Small plasmids (4.3 and 5.8 kb) were transduced at a frequency of 10(-5) to 10(-7)/PFU. However, we could not detect the transduction of any indigenous virulence plasmid (ca. 72 kb) in pathogenic Yersinia strains. Transductants obtained by infection with the temperate phage were lysogenic and harbored the phage genome in their chromosomes.

SUBMITTER: Hertwig S

PROVIDER: S-EPMC99712 | biostudies-literature | 1999 Sep

REPOSITORIES: biostudies-literature

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Generalized transduction of small Yersinia enterocolitica plasmids.

Hertwig S S Popp A A Freytag B B Lurz R R Appel B B

Applied and environmental microbiology 19990901 9

To study phage-mediated gene transfer in Yersinia, the ability of Yersinia phages to transduce naturally occurring plasmids was investigated. The transduction experiments were performed with a temperate phage isolated from a pathogenic Yersinia enterocolitica strain and phage mixtures isolated from sewage. Small plasmids (4.3 and 5.8 kb) were transduced at a frequency of 10(-5) to 10(-7)/PFU. However, we could not detect the transduction of any indigenous virulence plasmid (ca. 72 kb) in pathoge ...[more]

PMID: 10473387

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