Ontology highlight
ABSTRACT:
SUBMITTER: Delye C
PROVIDER: S-EPMC99725 | biostudies-literature | 1999 Sep
REPOSITORIES: biostudies-literature
Délye C C Ronchi V V Laigret F F Corio-Costet M F MF
Applied and environmental microbiology 19990901 9
Isolates of the obligately biotrophic fungus Uncinula necator cluster in three distinct genetic groups (groups I, II, and III). We designed PCR primers specific for these groups in order to monitor field populations of U. necator. We used the nucleotide sequences of the gene that encodes eburicol 14alpha-demethylase (CYP51) and of the ribosomal DNA internal transcribed spacer 1 (ITS1), ITS2, and 5. 8S regions. We identified four point mutations (three in CYP51 and one in ITS1) that distinguished ...[more]