Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase.
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ABSTRACT:
SUBMITTER: Beneke T
PROVIDER: S-EPMC9975418 | biostudies-literature | 2022
REPOSITORIES: biostudies-literature
Publications
Genome sequence of <i>Leishmania mexicana</i> MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase.
Beneke Tom T Dobramysl Ulrich U Catta-Preta Carolina Moura Costa CMC Mottram Jeremy Charles JC Gluenz Eva E Wheeler Richard J RJ
Wellcome open research 20220101
We present the genome sequence of <i>Leishmania mexicana</i> MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging. ...[more]
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