Ontology highlight
ABSTRACT:
SUBMITTER: Kweon J
PROVIDER: S-EPMC9981745 | biostudies-literature | 2023 Feb
REPOSITORIES: biostudies-literature
Kweon Jiyeon J Jang An-Hee AH Kwon Eunji E Kim Ungi U Shin Ha Rim HR See Jieun J Jang Gayoung G Lee Chaeyeon C Koo Taeyoung T Kim Seokjoong S Kim Yongsub Y
Experimental & molecular medicine 20230201 2
Various CRISPR‒Cas9 orthologs are used in genome engineering. One of the smallest Cas9 orthologs is cjCas9 derived from Campylobacter jejuni, which is a highly specific genome editing tool. Here, we developed cjCas9-based base editors including a cytosine base editor (cjCBEmax) and an adenine base editor (cjABE8e) that can successfully induce endogenous base substitutions by up to 91.2% at the HPD gene in HEK293T cells. Analysis of the base editing efficiency of 13 endogenous target sites showed ...[more]