Sub-tomogram averaging in RELION
Ontology highlight
ABSTRACT:
SUBMITTER: Tanmay AM Bharat
PROVIDER: EMPIAR-10045 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Resolving macromolecular structures from electron cryo-tomography data using subtomogram averaging in RELION.
Bharat Tanmay A M TA Scheres Sjors H W SH
Nature protocols 20160929 11
Electron cryo-tomography (cryo-ET) is a technique that is used to produce 3D pictures (tomograms) of complex objects such as asymmetric viruses, cellular organelles or whole cells from a series of tilted electron cryo-microscopy (cryo-EM) images. Averaging of macromolecular complexes found within tomograms is known as subtomogram averaging, and this technique allows structure determination of macromolecular complexes in situ. Subtomogram averaging is also gaining in popularity for the calculatio ...[more]
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