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Volta phase plate in-focus dataset of T20S proteasome

ABSTRACT:

SUBMITTER: Radostin Danev

PROVIDER: EMPIAR-10057 | biostudies-other |

REPOSITORIES: biostudies-other

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Cryo-EM single particle analysis with the Volta phase plate.

Danev Radostin R Baumeister Wolfgang W

eLife 20160307

We present a method for in-focus data acquisition with a phase plate that enables near-atomic resolution single particle reconstructions. Accurate focusing is the determining factor for obtaining high quality data. A double-area focusing strategy was implemented in order to achieve the required precision. With this approach we obtained a 3.2 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome. The phase plate matches or slightly exceeds the performance of the conventional ...[more]

PMID: 26949259

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