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Subset of image stack used for 3D reconstruction


ABSTRACT:

SUBMITTER: Edward Herschel Egelman 

PROVIDER: EMPIAR-10060 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

Virology. A virus that infects a hyperthermophile encapsidates A-form DNA.

DiMaio Frank F   Yu Xiong X   Rensen Elena E   Krupovic Mart M   Prangishvili David D   Egelman Edward H EH  

Science (New York, N.Y.) 20150501 6237


Extremophiles, microorganisms thriving in extreme environmental conditions, must have proteins and nucleic acids that are stable at extremes of temperature and pH. The nonenveloped, rod-shaped virus SIRV2 (Sulfolobus islandicus rod-shaped virus 2) infects the hyperthermophilic acidophile Sulfolobus islandicus, which lives at 80°C and pH 3. We have used cryo-electron microscopy to generate a three-dimensional reconstruction of the SIRV2 virion at ~4 angstrom resolution, which revealed a previousl  ...[more]

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