2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor
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ABSTRACT:
SUBMITTER: Sriram Subramaniam
PROVIDER: EMPIAR-10061 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor.
Bartesaghi Alberto A Merk Alan A Banerjee Soojay S Matthies Doreen D Wu Xiongwu X Milne Jacqueline L S JL Subramaniam Sriram S
Science (New York, N.Y.) 20150507 6239
Cryo-electron microscopy (cryo-EM) is rapidly emerging as a powerful tool for protein structure determination at high resolution. Here we report the structure of a complex between Escherichia coli β-galactosidase and the cell-permeant inhibitor phenylethyl β-D-thiogalactopyranoside (PETG), determined by cryo-EM at an average resolution of ~2.2 angstroms (Å). Besides the PETG ligand, we identified densities in the map for ~800 water molecules and for magnesium and sodium ions. Although it is like ...[more]
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