VPP subtomogram averaging
Ontology highlight
ABSTRACT:
SUBMITTER: Radostin Danev
PROVIDER: EMPIAR-10064 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Subtomogram analysis using the Volta phase plate.
Khoshouei Maryam M Pfeffer Stefan S Baumeister Wolfgang W Förster Friedrich F Danev Radostin R
Journal of structural biology 20160525 2
Cryo-electron tomography (CET) and subtomogram analysis allow studying the structures of macromolecular complexes in their natural context. The radiation sensitivity of vitrified biological specimens and the resulting low signal-to-noise ratio (SNR) in CET limit the amount of structural information that can be mined from tomographic data. The Volta phase plate (VPP) has emerged as an effective means to increase the SNR and hence contrast compared to 'conventional' defocus-based phase contrast tr ...[more]
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