Dataset Information
Untilted Single-Particle CryoEM of Highly Preferred Orientated Influenza Hemagglutinin Trimer
Ontology highlight
ABSTRACT:
SUBMITTER: Yong Zi Tan
PROVIDER: EMPIAR-10096 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Addressing preferred specimen orientation in single-particle cryo-EM through tilting.
Nature methods 20170703 8
We present a strategy for tackling preferred specimen orientation in single-particle cryogenic electron microscopy by employing tilts during data collection. We also describe a tool to quantify the resulting directional resolution using 3D Fourier shell correlation volumes. We applied these methods to determine the structures at near-atomic resolution of the influenza hemagglutinin trimer, which adopts a highly preferred specimen orientation, and of ribosomal biogenesis intermediates, which adop ...[more]