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Cryo-ET of natural chromatin from Ostreococcus tauri and Saccharyomyces cerevisiae

ABSTRACT:

SUBMITTER: Lu Gan

PROVIDER: EMPIAR-10098 | biostudies-other |

REPOSITORIES: biostudies-other

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Natural chromatin is heterogeneous and self-associates in vitro.

Cai Shujun S Song Yajiao Y Chen Chen C Shi Jian J Gan Lu L

Molecular biology of the cell 20180509 13

The 30-nm fiber is commonly formed by oligonucleosome arrays in vitro but rarely found inside cells. To determine how chromatin higher-order structure is controlled, we used electron cryotomography (cryo-ET) to study the undigested natural chromatin released from two single-celled organisms in which 30-nm fibers have not been observed in vivo: picoplankton and yeast. In the presence of divalent cations, most of the chromatin from both organisms is condensed into a large mass in vitro. Rare irreg ...[more]

PMID: 29742050

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