Dataset Information
CryoET of apoferritin with 0.5 mM TCEP single particle
Ontology highlight
ABSTRACT:
SUBMITTER: Bridget Carragher
PROVIDER: EMPIAR-10141 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Routine single particle CryoEM sample and grid characterization by tomography.
eLife 20180529
Single particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are not adsorbed to the air-water interfaces and in thin, vitreous ice. In this study, we performed fiducial-less tomography on over 50 different cryoEM grid/sample preparations to determine the particle distribution within the ice and the overall geometry of the ice in grid holes. Surprisingly, by studying particles in holes in 3D from over 1000 tomograms, we have determined that the vast ma ...[more]