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Volta phase plate data collection facilitates image processing and cryo-EM structure determination

ABSTRACT:

SUBMITTER: Bruno P Klaholz

PROVIDER: EMPIAR-10154 | biostudies-other |

REPOSITORIES: biostudies-other

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Volta phase plate data collection facilitates image processing and cryo-EM structure determination.

von Loeffelholz Ottilie O Papai Gabor G Danev Radostin R Myasnikov Alexander G AG Natchiar S Kundhavai SK Hazemann Isabelle I Ménétret Jean-François JF Klaholz Bruno P BP

Journal of structural biology 20180111 3

A current bottleneck in structure determination of macromolecular complexes by cryo electron microscopy (cryo-EM) is the large amount of data needed to obtain high-resolution 3D reconstructions, including through sorting into different conformations and compositions with advanced image processing. Additionally, it may be difficult to visualize small ligands that bind in sub-stoichiometric levels. Volta phase plates (VPP) introduce a phase shift in the contrast transfer and drastically increase t ...[more]

PMID: 29337113

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