Cryo electron tomography of immotile sea urchin sperm flagella
Ontology highlight
ABSTRACT:
SUBMITTER: Daniela Nicastro
PROVIDER: EMPIAR-10158 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Asymmetric distribution and spatial switching of dynein activity generates ciliary motility.
Lin Jianfeng J Lin Jianfeng J Nicastro Daniela D
Science (New York, N.Y.) 20180401 6387
Motile cilia and flagella are essential, highly conserved organelles, and their motility is driven by the coordinated activities of multiple dynein isoforms. The prevailing "switch-point" hypothesis posits that dyneins are asymmetrically activated to drive flagellar bending. To test this model, we applied cryo-electron tomography to visualize activity states of individual dyneins relative to their locations along beating flagella of sea urchin sperm cells. As predicted, bending was generated by ...[more]
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