Dataset Information
Single particle cryoEM of hemagglutinin with spot-to-plunge time of 500ms
Ontology highlight
ABSTRACT:
SUBMITTER: Bridget Carragher
PROVIDER: EMPIAR-10197 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Reducing effects of particle adsorption to the air-water interface in cryo-EM.
Nature methods 20180924 10
Most protein particles prepared in vitreous ice for single-particle cryo-electron microscopy (cryo-EM) are adsorbed to air-water or substrate-water interfaces, which can cause the particles to adopt preferred orientations. By using a rapid plunge-freezing robot and nanowire grids, we were able to reduce some of the deleterious effects of the air-water interface by decreasing the dwell time of particles in thin liquid films. We demonstrated this by using single-particle cryo-EM and cryo-electron ...[more]