Separating distinct macromolecular assemblies from cryo-EM images
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ABSTRACT:
SUBMITTER: David W. Taylor
PROVIDER: EMPIAR-10268 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Separating distinct structures of multiple macromolecular assemblies from cryo-EM projections.
Verbeke Eric J EJ Zhou Yi Y Horton Andrew P AP Mallam Anna L AL Taylor David W DW Marcotte Edward M EM
Journal of structural biology 20191111 1
Single particle analysis for structure determination in cryo-electron microscopy is traditionally applied to samples purified to near homogeneity as current reconstruction algorithms are not designed to handle heterogeneous mixtures of structures from many distinct macromolecular complexes. We extend on long established methods and demonstrate that relating two-dimensional projection images by their common lines in a graphical framework is sufficient for partitioning distinct protein and multipr ...[more]
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