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Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome

ABSTRACT:

SUBMITTER: Jean-Paul Armache

PROVIDER: EMPIAR-10287 | biostudies-other |

REPOSITORIES: biostudies-other

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Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome.

Armache Jean Paul JP Gamarra Nathan N Johnson Stephanie L SL Leonard John D JD Wu Shenping S Narlikar Geeta J GJ Cheng Yifan Y

eLife 20190618

The SNF2h remodeler slides nucleosomes most efficiently as a dimer, yet how the two protomers avoid a tug-of-war is unclear. Furthermore, SNF2h couples histone octamer deformation to nucleosome sliding, but the underlying structural basis remains unknown. Here we present cryo-EM structures of SNF2h-nucleosome complexes with ADP-BeF<sub>x</sub> that capture two potential reaction intermediates. In one structure, histone residues near the dyad and in the H2A-H2B acidic patch, distal to the active ...[more]

PMID: 31210637

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Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome.

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