PolyA polymerase module of the cleavage and polyadenylation factor (CPF) from Saccharomyces cerevisiae
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ABSTRACT:
SUBMITTER: Passmore LA
PROVIDER: EMPIAR-10299 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Architecture of eukaryotic mRNA 3'-end processing machinery.
Casañal Ana A Kumar Ananthanarayanan A Hill Chris H CH Easter Ashley D AD Emsley Paul P Degliesposti Gianluca G Gordiyenko Yuliya Y Santhanam Balaji B Wolf Jana J Wiederhold Katrin K Dornan Gillian L GL Skehel Mark M Robinson Carol V CV Passmore Lori A LA
Science (New York, N.Y.) 20171026 6366
Newly transcribed eukaryotic precursor messenger RNAs (pre-mRNAs) are processed at their 3' ends by the ~1-megadalton multiprotein cleavage and polyadenylation factor (CPF). CPF cleaves pre-mRNAs, adds a polyadenylate tail, and triggers transcription termination, but it is unclear how its various enzymes are coordinated and assembled. Here, we show that the nuclease, polymerase, and phosphatase activities of yeast CPF are organized into three modules. Using electron cryomicroscopy, we determined ...[more]
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2024-09-15 | GSE242763 | GEO